Fig 1: Condition optimization of intermediate mesoderm induced by human induced pluripotent stem cells. (A) Bright field observations at day 4 of induction by gradient concentration of CHIR99021. (B) RT-qPCR detection of the primitive streak markers T, TBX6 and LHX1 at day 4 of induction. (C) RT-qPCR detection of anterior intermediate mesoderm marker GATA3, and posterior intermediate mesoderm markers HOXD11 and EYA1, at day 4 of induction. The data are representative from a minimum of three independent experiments. RT-qPCR, reverse transcription-quantitative PCR; TBX6, T-box transcription factor 6; LHX1, LIM homeobox 1; GATA3, GATA binding protein 3; HOXD11, homeobox 11; EYA1, EYA transcriptional coactivator and phosphatase 1; FGF9, fibroblast growth factor 9.